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The Human SEMA4D (Semaphorin 4D) ELISA kit uses the Sandwich-ELISA principle
And then biotinylated Human ABL2 Antibody is added and binds to ABL2 in the sample
Only those wells that contain Human SDF-1/CXCL12
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EPOR
Rat ANXA2 (Annexin A2) ELISA Kit Packaging:96 wells The Human SEMA4D (Semaphorin 4D)The Rat (ANXA2) Annexin A2 ELISA Kit measures Annexin A2 in Rat samples. The plate has been pre coated with Rat ANXA2 antibody. ANXA2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ANXA2 Antibody is added and binds to ANXA2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ANXA2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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